A model of the acid sphingomyelinase phosphoesterase domain based on its remote structural homolog purple acid phosphatase

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A model of the acid sphingomyelinase phosphoesterase domain based on its remote structural homolog purple acid phosphatase.

Sequence profile and fold recognition methods identified mammalian purple acid phosphatase (PAP), a member of a dimetal-containing phosphoesterase (DMP) family, as a remote homolog of human acid sphingomyelinase (ASM). A model of the phosphoesterase domain of ASM was built based on its predicted secondary structure and the metal-coordinating residues of PAP. Due to the low sequence identity bet...

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Functional Assessment of an Overexpressed Arabidopsis Purple Acid Phosphatase Gene (Atpap26) in Tobacco Plants

Background: Overexpression of known genes encoding key phosphate (Pi)-metabolizing enzymes, such as acid phosphatases (APases), is presumed to help plants with Pi availability and absorption as they are mostly exposed to suboptimal environmental conditions for this vital element.Objectives: In this study, the overexpression effect of AtPAP26, one of the m...

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Low-phosphorus (P) stress as a key factor limiting plant growth and production is common in most agricultural soils. Most of the soil-applied phosphate will be rapidly immobilized and most of annually applied phosphate fertilizers are fixed in the soil in organic forms by adsorption, sedimentation and transformation. However, excess P application may lead to contamination of water sources by en...

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ژورنال

عنوان ژورنال: Protein Science

سال: 2009

ISSN: 0961-8368

DOI: 10.1110/ps.04966204